Download pdf info publication number us20190002848a1. There are three types of pkss type i, type ii, and type iii in streptomyces responsible for producing polyketides. Ever since the initial description of modular type i polyketide synthases. The shikimic acid pathway and polyketide biosynthesis. Typically the products of microbial secondary biosynthesis, polyketides are synthesized by an evolutionarily related but architecturally diverse family of multifunctional enzymes called polyketide synthases. The module detects genesproteins in the type ii pks gene cluster involved with polyketide biosynthesis and is able to make predictions about the aromatic polyketide. The shikimic acid pathway, ubiquitous in microorganisms and plants, provides precursors for the biosynthesis of primary metabolites such as the aromatic amino acids and folic acid. In modular pkss, the polyketide chain is assembled by the successive condensation of activated carboxylic acidderived units, where chain. The orfvi from the cluster of genes, which is responsible for the biosynthesis of the streptomyces coelicolor spore pigment, the whie cluster, has been described as a bifunctional aromatasecyclase. Pdf programmable polyketide biosynthesis platform for. Thus, although the legoization of polyketide combinatorial biosynthesis has hit its stride, many pieces.
Summary polyketide natural products show great promise as medicinal agents. Polyketide biosynthesis is catalyzed by polyketide synthase pks and three types of bacterial pks are. The stereochemistry of complex polyketide biosynthesis. A principal limitation for fundamental biochemical studies of these modular megasynthases, as well as for their. Biosynthesis of the yellow xanthomonadin pigments involves. View enhanced pdf access article on wiley online library html view download pdf for offline viewing. Acyl carrier proteins acps are essential to both fatty acid synthase fas and polyketide synthase pks biosynthetic pathways, yet relatively little is known about how they function at a. The platform is based on a synthetic polyketide synthase system enabling a first demonstration of bacterial aromatic polyketide biosynthesis in a eukaryotic host. A methylation of the acetate starter unit is part of the polyketide biosynthesis of phthalides and phthalimidines from the marinederived fungus stachylidium sp.
This paper focuses on the biosynthesis of polyketides in streptomyces with three structurallydifferent types of pkss. New insights into bacterial type ii polyketide biosynthesis. Structure of chalcone synthase and the molecular basis of. Polyketides are a diverse class of medically important natural products whose biosynthesis is catalysed by polyketide synthases pkss, in a fashion highly analogous to fatty acid biosynthesis.
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